Estudo de isolados clínicos e ambientais de Fusarium solani: perfil de sensibilidade a antifúngicos em combinação e de fatores associados à virulência / Study of clinical and environmental isolates of Fusarium solani: sensitivity profile to antifungal agents in combination and of factors associated with virulence

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Despite of the increase of opportunistic fungal infections caused by Fusarium spp. and their resistance to antifungal drugs, the aims of this study were to evaluate susceptibility profiles of 20 clinical and environmental isolates of Fusarium solani against the associations among terbinafine, itraconazole, ketoconazole and amphotericin B, and to verify the presence of associated virulence factors as melanin and extracellular enzymes (proteinase, phospholipase, lipase and DNAse). Amphotericin B combined with itraconazole showed synergism against 15% of the isolates, 33% of these was from the environment and 65% from clinical cases. Synergistic effect was observed against 10% of strains tested by association between amphotericin B and ketoconazole and against 5% in association between anphotericin B and terbinafine. The associations between terbinafine and azole derivatives showed additive effect and no difference against the samples. Presence of melanin in the F. solani cell wall was not observed. Both, clinical and environmental isolates expressed enzymatic activity of proteinase in 30% and 45% of cases. Enzymatic activity of lipase, phospholipase and DNAse were not detected; however, it was not possible to state that F. solani do not produce these enzymes, as they could manteined intracellularly and would not released to the environment.

ASSUNTO(S)

fusarium solani combinação antifúngicos enzimas extracelulares melanina virulência dermatologia fusarium solani antifungals association extracellular enzymes melanin virulence

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