Estudo do metodo de utilização do glicol metacrilato como material de inclusão de implantes subcutaneos em ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

One of the most practical and widely used methods to assess the biocompatibility of endodontic sealers is the implantation of polyethylene tubes filled with the sealer into the subcutaneous connective tissue of rats. The irritant effect of the sealers is then evaluated by the histological process of the tubes with surrounding tissue, usually using paraffin embedding, presents several technical difficulties wich can influence in the analysis of the results. The aim of this work was to study the efficence of glycol methacrylate as embedding material of rat subcutaneous tissue wich received implants of tubes filled with endodontic sealer. Polyethylene tubes hot sealed in one end and were filled with endodontic sealer (Endomethasone) were implanted into subcutaneous tissue of rats. After the 30 days, the animais were killed and blocks with epithelium tissue, tube and sealer, connective tissue and muscular tissue were removed and fixed in Bouin to 24 hours and in 10% buffered formalin until being processed. The tubes were then transversally cut to higher axis, the sealed part was despised ando the other one cut in the midle of the higher axis, this way we had two similar halves. One of the halves was process~d by paraffin embedding method and the other one by glycol methacrylate methad. The results acheived when the glycol methacrylate was used were highly superior than the ones with paraffin, showing a good histologic quality wich allowed an improved analysis of cell an tissues biology with ligth microscopy. Therefore, it is concluded that the glycol methacrylate is as alternative and efficent method to assess the biocompatibility of endodontic sealers into subcutaneous tissue of rats

ASSUNTO(S)

biocompatibilidade endodontia cimentos dentarios

ACESSO AO ARTIGO

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