Estudo do perfil da expressão genica global em leucemias linfoides agudas de linhagens de celulas B e T
AUTOR(ES)
Diana Azevedo Queiroz
DATA DE PUBLICAÇÃO
2004
RESUMO
The comprehension of data generated by many finished or in progress Genome Projects will provide essential informations to molecular biology research. The identification of differentially expressed genes in distinct stages of any given disease, for example, will permit an enormous advance in the determination of its physiopathology, identifying molecular markers for its prognostic and possible targets for the intervention of the process. Among human diseases, cancer consists in one of the main subjects of research in clinical and molecular biology, basically because involves a complex and poorly understood process of cellular reprogramming. Among the cancer subtypes, acute lymphoblastic leukemia is prevalent in Brazil, ranked in 8th position in number of clinical cases and decorrent deaths. Attempting to increase the understanding of cancer molecular bases, Human Cancer Genome Project (LICR/ FAPESP) had detached by generation of thousand of expressed sequences of normal and tumoral human tissues, increasing the possibility of discovery of novel genes related to carcinogenic processes. Thus, the present work employed the Human Cancer Genome Project fragments to investigate the global gene expression profile in acute lymphoblastic leukemia by DNA microarrays technique. It was identified the genes of IgK VLJ (imunoglobulina kappa light chain VLJ region) e TPT1 (Tumor Protein, ananslationally-controlled 1), which differential expression was confirmed by Northern blot assays.
ASSUNTO(S)
celulas expressão genica bioquimica
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=vtls000343518Documentos Relacionados
- Diagnóstico laboratorial das leucemias linfóides agudas
- Estudo de elementos moduladores da expressão gênica em diferentes linhagens de células de mamífero em cultura
- Expressão do gene MRP1 em leucemias agudas
- Mutações de PTEN nas leucemias linfóides agudas T
- Perfil de expressão genica de leucemias agudas por tecnica de microarranjo (microarrays)