Estudo histolÃgico comparativo entre autoimplantes esplÃnicos e a porÃÃo nÃo-implantada do baÃo de camundongos sadios e na fase crÃnica da esquistossomose mansÃnica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

In order to reduce the adverse immunological effects of surgical asplenic state, it has been proposed to preserve as much splenic tissue as possible. In situations when the removal of the whole organ is unavoidable, splenic autoimplantation proved to be a good alternative for this purpose. The steps of structural reestablishment of the tissue have been histologically shown in healthy individuals who undergone this procedure. The purpose of this study was to compare patterns, under the perspective of histological regeneration, between the splenic autoimplants and the removed and not implanted portion of spleen, both of healthy and chronically schistosomotic mice. Thirty two adult female mice of non-isogenic strains were used in the study, 16 of them previously infected with cercariae of S. mansoni. The surgical procedure consisted of total splenectomy, followed by the implant of 1/2 of the whole spleen sliced into an omental pouch in the schistosomotic animals, and of about 2/3 of the organ in the healthy ones. Eight weeks after operations, all animals were sacrificed. Four subgroups were analyzed: the not implanted portion of the spleen; in healthy mice (S1), and in the chronic stage of schistosomiasis (E1) â the splenic autoimplants; in healthy animals (S2), and in those in the chronic stage of disease (E2). Histological analysis of splenic autoimplants into the greater omentum showed complete regeneration in all animals, similar to the microscopic structure of the ordinary spleen. The differences found in autoimplants compared to the not implanted portion of the spleen were, basically, the alterations related to the proliferation of fibroconnective tissue, such as: greater thickness of the new formed capsule, more interstitial fibrosis and a higher number of megakaryocytes and olimorphonuclear leucocytes in the red pulp. There was a higher expression of large and medium lymphocytes in the germinative centers of the not implanted portion, compared to the splenic autoimplants of infected mice. Comparison with subgroups of healthy animals showed that extrafollicular lymphocytes increased in number in the splenic autoimplants. Differences between healthy animals and those with schistosomiasis were due to parenquimal changes caused by schistosomotic disease and by a higher number of brown pigment and plasma cells. There was no difference in number, localization and antigenic material present in the splenic schistosomotic granulomas, both in not implanted portion of the spleen and autoimplants

ASSUNTO(S)

enxertos â baÃo â esquistossomose mansÃnica (fase crÃnica) â estudo experimental anatomia patologica e patologia clinica histologia â auto-implantes â baÃo (porÃÃo nÃo-implantada) â estudo comparativo baÃo â auto-implantes â camundongos â estudo histolÃgico autoimplantes esplÃnicos â camundongos â estudo histolÃgico â regeneraÃÃo esquistossomose mansÃnica â auto-implantes â estudo experimental (microscopia Ãptica)

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