Estudos da inflamação e dor articulares em ratos e dos mecanismos da produção de TNF-a por macrófagos isolados, induzidos pela BaP1, uma metaloproteínase isolada do veneno da serpente Bothrops asper. / Studies on both rat articular inflammation and pain, and mechanisms involved in production of TNF-a by isolated macrophages induced by BaP1, a metalloproteinase isolated from Bothrops asper snake venom.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Metalloproteinases are major enzymes in snake venoms showing high grade of homology with mammal matrix metalloproteinases, present in high levels in inflamed joints. In this study we examined the ability of BaP1, to induce: i) inflammation and hypernociception in rat articular joints and participation of TNF-a and PGE2 in these effects, and ii) activation of cultured macrophages. BaP1 increased vascular permeability, induced release of TNF-a, PGE2 and pro-MMP-9 in joint cavities, and leucocyte influx into joint cavities and synovial tissues. Moreover, BaP1 induced articular hypernociception. Treatment of animals with indomethacin or antiserum anti-TNF-a significantly reduced hypernociception and leukocyte influx induced by BaP1. Incubation of macrophages with BaP1 caused expression of TNF-a and COX-2 as well as TNF-a release. In conclusion, BaP1 induces inflammation and hypernociception in articular joints. These effects are dependent on PGE2 and TNF-a. COX-2 may contribute for BaP1-induced PGE2 release and macrophages are key targets for BaP1 induced effects.

ASSUNTO(S)

inflammation metaloproteinase artrite dor metalloproteinase macrófagos macrophages inflamação arthritis pain

ACESSO AO ARTIGO

Documentos Relacionados