Estudos genÃtico/fisiolÃgicos dos mutantes alcobaÃa (alc), non ripening (nor), e ripening inhibitor (rin) em tomateiro / Genetic-Physiological studies of the tomato mutants alcobaÃa (alc), non-ripening (nor) e ripening-inhibitor (rin).

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The tomato mutants alcobaÃa (alc), non-ripening (nor) and ripening-inhibitor (rin) were compared for their post-harvest effects as homozygotes, as heterozygotes and in their double heterozygous combinations. The genotypes Flora-Dade, TOM-559 (alc/alc), TOM-613 (nor/nor), TOM-614 (rin/rin), F1 [(Flora-Dade x TOM-613)] (nor+/nor), F1 [(Flora-Dade x TOM-559)] (alc+/alc), F1 [(Flora-Dade x TOM-614)] (rin+/rin), F1 [(TOM-559 x TOM-613)] (alc+/alc nor+/nor), F1 [(TOM-559 x TOM-614)] (alc+/alc rin+/rin) and F1 [(TOM-613 x TOM-614)] ( nor+/nor rin+/rin), were compared for their effects on the evolution of fruit colour and on the decrease of fruit firmness. Among the homozytes, rin/rin had the most drastic effects in delaying firmness loss and evolution of fruit colour. Among the heterozytes, rin+/rin was more effective than either alc+/alc or nor+/nor; rin+/rin. The double heterozygous combinations alc+/alc rin+/rin and nor+/nor rin+/rin had similar effects. A second experiment evaluated final fruit colour of the genotypes Flora-Dade, TOM-559, TOM-613, F1 (TOM-559 x TOM-613), F1 (Flora-Dade x TOM-559), F1 (Flora-Dade x TOM-613) and the F2 [(TOM-559 x TOM-613)] generation. No recombinant normal phenotypes were detected among 364 F2 plants tested. The hypothesis that alc and nor are alleles in the same locus was considered the most likely. A third experiment carried out at the Brazilian Maize Sorghum Research, located at Sete Lagoas, Minas Gerais State, five almost isogenic tomato lines, Flora-Dade, TOM-559 (alc alc ), TOM-595 (hp hp),TOM-596 (ogc ogc) and TOM-613 (nor nor) were genotyped using 119 RAPD molecular markers. The main objective was to identify molecular markers associated to alc (alcobaÃa), nor (non-ripening), hp (high pigment) and ogc (old gold crimson) alleles. The results did not show any polymorphism even for the three primers previously shown to been linked to the hp allele.

ASSUNTO(S)

lycopersicum esculentum teste de alelismo mutantes de amadurecimento alelism test lycopersicum esculentum melhoramento vegetal rapd rapd conservaÃÃo pÃs-colheita post-harvest conservation rippening mutants

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