Ethanol Extraction Requirement for Purification of Protein Labeled with [3H]Leucine in Aquatic Bacterial Production Studies †
AUTOR(ES)
Wicks, Richard J.
RESUMO
The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [3H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [3H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204452Documentos Relacionados
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