Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
AUTOR(ES)
Tan, Timothy H. P.
FONTE
The National Academy of Sciences
RESUMO
The mitochondrion of Trypanosoma brucei lacks tRNA genes. Its translation system therefore depends on the import of cytosolic, nucleus-encoded tRNAs. Thus, most trypanosomal tRNAs function in both the cytosol and the mitochondrion, and all are of the eukaryotic type. This is also the case for the elongator tRNAMet, whereas the only other trypanosomal tRNAMet, the eukaryotic initiator, is found exclusively in the cytosol. Unlike their cytosolic counterparts, organellar initiator tRNAsMet carry a formylated methionine. This raises the question of how initiation of translation works in trypanosomal mitochondria, where only elongator tRNAMet is found. Using in organello charging and formylation assays, we show that unexpectedly a fraction of elongator tRNAMet becomes formylated after import into mitochondria. Furthermore, in vitro experiments with mitochondrial extracts demonstrate that only the trypanosomal elongator and not the initiator tRNAMet is recognized by the formylation activity. Finally, RNA interference assays identify the gene encoding the trypanosomal formylase activity. Whereas the predicted protein is homologous to prokaryotic and mitochondrial methionyl-tRNAMet formyltransferases, it has about twice the mass of any of these proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=122159Documentos Relacionados
- Location alters tRNA identity: Trypanosoma brucei's cytosolic elongator tRNAMet is both the initiator and elongator in mitochondria
- Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo.
- Nucleotide sequence of chloroplast CF1-ATPase epsilon-subunit and elongator tRNAMet genes from Arabidopsis thaliana.
- Complementary DNAs encoding eukaryotic-type cytidine-5'-diphosphate-diacylglycerol synthases of two plant species.
- Nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC): the tRNAVal (UAC) gene contains a long intron.
