Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

AUTOR(ES)

Izquierdo, Luis, Marques, Alexandre Ferreira, Gállego, Montserrat, Sanz, Sílvia, Tebar, Sílvia, Riera, Cristina, Quintó, Llorenç, Aldasoro, Edelweiss, Almeida, Igor C, Gascon, Joaquim

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

2013-11

RESUMO

The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.

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