Evaluation of a Fluorescent Lectin-Based Staining Technique for Some Acidophilic Mining Bacteria
AUTOR(ES)
Fife, Dee Jay
FONTE
American Society for Microbiology
RESUMO
A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245–2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=101474Documentos Relacionados
- Alternate gram staining technique using a fluorescent lectin.
- A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of Thiobacillus ferrooxidans in a Population of Acidophilic Bacteria
- Rapid Fluorescent-Antibody Staining Technique
- A Flow-Cytometric Gram-Staining Technique for Milk-Associated Bacteria
- AN IMPROVED TECHNIQUE FOR STAINING RED HALOPHILIC BACTERIA