Evaluation of Helicobacter pylori genotype in the oral cavity / Pesquisa da cepa de Helicobacter pylori  na cavidade bucal

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Helicobacter pylori (H. pylori) infection is very prevalent in Brazil, infecting almost 65% of our population, being associated with peptic ulcer disease, gastric cancer, lymphoma and functional dyspepsia. The aim of this study was to evaluate the presence of this bacterium in the oral cavity of epigastric pain syndrome (functional dyspepsia) patients and assess the frequency of cagA and vacA genotypes of oral H. pylori. All 43 epigastric pain syndrome outpatients, who entered the study, were submitted to upper gastrointestinal endoscopy to rule out organic diseases. H. pylori infection was confirmed by rapid urease test, urea breath test and sorology, and thirty patients harbored H. pylori in the stomach. 144 samples of the oral cavity were collected: 43 samples of saliva, 43 of the tongue dorsum and 43 of supragingival dental plaque and 15 of sub-gingival dental plaque which were collected from the patients with periodontitis; H. pylori infection was verified by polymerase chain reaction (PCR) using primers (P1 and P2) that amplify the DNA sequence of a species-specific antigen present in all H. pylori strains, primers Urease A/B and primers for cagA and vacA (m1, m2, s1a, s1b, s2) genotyping. It was not possible to detect H. pylori in any oral samples using P1/P2 and Urease A/B. The genotype cagA was also negative in all samples and vacA genotype could not be characterized (s-m-). In this study, the oral cavity seemed not to be a reservoir for H. pylori in patients with epigastric pain syndrome being detected exclusively in the stomach.

ASSUNTO(S)

placa dentária saliva dyspepsia boca dental plaque mouth helicobacter pylori helicobacter pylori polymerase chain reaction dispepsia saliva reação em cadeia da polimerase

ACESSO AO ARTIGO

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