Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics
AUTOR(ES)
Santos, R.L., Pithon, M.M., Pereira, A.R.B., Romanos, M.T.V.
FONTE
Matéria (Rio J.)
DATA DE PUBLICAÇÃO
2012
RESUMO
Allergy caused by latex proteins has been well documented. Thus, the study of non-latex materials, is necessary. The aim of this study is to evaluate the cytotoxicity of silver-coloured orthodontic chain elastics, of polyurethane and latex-free. Nine chain elastics from different manufactures (3 latex-free and 6 polyurethane) were divided into 9 groups of 10 elastics each: Group UK (Latex-free, 3M Unitek), Group TP (Látex-free, TP Orthodontics), Group AO (Látex-free, American Orthodontics), Group O (Polyurethane, OrthoSource), Group M (Polyurethane, Morelli), Group TD (Polyurethane, Tecnident), Group UD (Polyurethane, Uniden), Group AZ (Polyurethane, Abzil) and Group AK (Polyurethane, Aditek). The cytotoxicity essay was performed using cell cultures (L-929 line cells, mouse fibroblast) that were submitted to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Analysis of variance (ANOVA) with multiple comparisons and Tukey's test were employed (p<.05). The results showed no statistically significant differences between Groups UK, TP and AO in all experimental times (p>.05), except between the Groups UK and TP at 28 days (p<.05). There was significant statistically difference (p<.05) between the viability of the cells in the Groups O and TD with the Groups M, AZ, AK at 1 and 2 days. The polyurethane elastics showed lower cell viability in the first 48 hours, with increase on 3rd and 7th day, and viability similar to latex-free elastics at 28 days. It can be concluded that the latex-free chain elastic showed higher cell viability. The OrthoSource and Tecnident trademark showed lower cell viability the initial 48 hours.
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