Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens.
AUTOR(ES)
Wu, T C
RESUMO
The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivity of 90% and a specificity of 99% compared with the shell vial tissue culture method which uses primary rabbit kidney cells. Five false-negative results by HERPCHEK were found with specimens that contained a low percentage of positive cells by the direct immunoperoxidase stain used in a tissue culture method (1 to 9%). The absorbance values of the clinical specimens by the HERPCHEK test do not correlate well with the percentage of positive cells. Our results indicate that the HERPCHEK HSV antigen test, when performed with specimens from lesions, is a sensitive and specific test as compared with shell vial culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267698Documentos Relacionados
- Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.
- Herpes simplex virus antigen direct detection in standard virus transport medium by Du Pont Herpchek enzyme-linked immunosorbent assay.
- Evaluation of an automated immunodiagnostic assay system for direct detection of herpes simplex virus antigen in clinical specimens.
- Evaluation of the virocult transport tube for isolation of herpes simplex virus from clinical specimens.
- Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.
