Evaluation of Two Standardized Disk Methods for Testing Antimicrobial Susceptibility of Pseudomonas aeruginosa and of the Enterobacteriaceae
AUTOR(ES)
Barry, Arthur L.
RESUMO
Two standardized disk diffusion susceptibility testing methods were compared using Pseudomonas aeruginosa and members of the Enterobacteriaceae. The standard Bauer-Kirby method and the agar overlay method of Barry et al. were compared using carbenicillin (50- and 100-μg disks), gentamicin, polymyxin B, and colistin sulfate. Both methods gave nearly comparable zone sizes with the enteric bacilli. However, with P. aeruginosa the agar overlay method gave zones 1 to 2 mm smaller than the Bauer-Kirby method with all drugs except gentamicin. In spite of these small differences, further examination of minimal inhibitory concentration-zone size correlations indicated that the same interpretive zone standards can be applied to either diffusion technique. For testing carbenicillin against Pseudomonas sp., 50-μg disks were unsatisfactory, especially with the agar overlay method; 100-μg disks were far superior with both methods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444670Documentos Relacionados
- Antimicrobial Susceptibility Testing of Carbapenems: Multicenter Validity Testing and Accuracy Levels of Five Antimicrobial Test Methods for Detecting Resistance in Enterobacteriaceae and Pseudomonas aeruginosa Isolates
- Evaluation of two broth disk methods for antibiotic susceptibility testing of anaerobes.
- Multicenter laboratory evaluation of the bioMérieux Vitek antimicrobial susceptibility testing system with 11 antimicrobial agents versus members of the family Enterobacteriaceae and Pseudomonas aeruginosa.
- Evaluation of Reference Dilution Test Methods for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Strains Isolated from Patients with Cystic Fibrosis
- Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa
