Evidence for a complex structure of neutralization antigenic site I of poliovirus type 1 Mahoney.
AUTOR(ES)
Wiegers, K
RESUMO
We have selected neutralization escape mutants by using a monoclonal antibody (nt-MAb) against a sequential epitope between amino acids 93 through 104 (neutralization antigenic site I) of poliovirus type 1 Mahoney. The majority of mutants were also resistant against five strain-specific nt-MAbs which recognized conformation-dependent epitopes, suggesting that the neutralization antigenic site I must be involved in the formation of such epitopes. An analysis of all mutants by the binding of nt-MAbs and by isoelectric focusing of VP1 allowed discrimination of five classes of mutants. Sequence analysis of mutant RNAs revealed point mutations and deletions in the antibody-binding site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253247Documentos Relacionados
- Evidence for a Complex Structure of Neutralization Antigenic Site I of Poliovirus Type 1 Mahoney
- Construction of a poliovirus type 1/type 2 antigenic hybrid by manipulation of neutralization antigenic site II.
- Identification of a T-cell epitope adjacent to neutralization antigenic site 1 of poliovirus type 1.
- Identification of a New Neutralization Antigenic Site on Poliovirus Coat Protein VP2
- Identification of a new neutralization antigenic site on poliovirus coat protein VP2.