EVIDENCE FOR THE DE NOVO SYNTHESIS OF THE ALPHA-AMYLASE OF PSEUDOMONAS SACCHAROPHILA

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Eisenstadt, Jerome M. (Brandeis University, Waltham, Mass.) and Harold P. Klein. Evidence for the de novo synthesis of the alpha-amylase of Pseudomonas saccharophila. J. Bacteriol. 82:798–807. 1961.—Chloramphenicol at a concentration of 20 μg per ml inhibited the appearance of the inducible α-amylase of Pseudomonas saccharophila. This inhibition was observed when induction was attempted in buffer or in a complete medium. Preinduced cells were also prevented from forming this enzyme under similar conditions. Under all the conditions tested, there was no lag in chloramphenicol inhibition, thus suggesting an absence of any protein precursor in amylase formation.

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