Evidence for unequal crossing over within the mouse T/t complex.
AUTOR(ES)
Silver, L M
RESUMO
The Tcp-1 gene located within the T/t complex on chromosome 17 of the mouse codes for a major cell surface-associated protein p63/6.9. Previously, we identified two structural alleles of this gene which specify alternate forms of the p63/6.9 protein. The Tcp-1b allele is associated with all wild-type chromosome 17; the Tcp-1a allele is found only with chromosome 17 carrying a complete t haplotype. Normal recombination along a major length of chromosome 17 is suppressed in mice that are heterozygous for any complete t haplotype. Suppression is not complete, however, and rare crossing over between wild-type and t haplotype chromatin does occur. In this report, 15 rare recombinant chromosomes have been analyzed for Tcp-1 alleles. The results indicate that in four independent events the Tcp-1b and Tcp-1a alleles have become associated in cis position in a single DNA molecule. Further genetic analysis provides support for the hypothesis that a significant nonhomology exists between the arrangement of DNA sequences on wild-type and t-carrying chromosome 17. This could account for both the suppression of normal recombination along the stretch of t chromatin and the frequent unequal crossing over when rare recombinational events do take place.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350216Documentos Relacionados
- Embryonal carcinoma antigen and the T/t locus of the mouse.
- A New Mutation (t-int) Interacts with the Mutations of the Mouse T/t Complex That Affect the Tail
- Homology Requirements for Unequal Crossing over in Humans
- Unequal Crossing over Then and Now
- Molecular mapping within the mouse albino-deletion complex.