Expressão de Cyanovirin-N, um Microbicida Anti-HIV, em Plantas

AUTOR(ES)

Luisa de Moraes Madeira

DATA DE PUBLICAÇÃO

2008

RESUMO

The Human immunodeficiency virus (HIV) is covered by an envelope from which proteins, specially the gp120, control its entering into susceptible cells. The HIV infection results in the Acquired Immunodeficiency Syndrome (AIDS), and in spite of the global efforts against its pandemic, the number of people with HIV increases every year. Heterosexual intercourse is currently the main way of transmission, which implicates on the growing number of infected women. Topical microbicides are products that can be applied to the vagina or rectum prior to intercourse to prevent the sexual transmission of HIV, and emerged as an additional tool directed for women to prevent AIDS. Cyanovirin-N (CV-N) is a microbicide candidate that can inactivate a wide range of HIV strains by binding irreversibly to gp120. Production of CV-N, or any microbicide, needs to be at extremely high levels to supply the demand, and genetically modified plants offer a suitable system. Transgenic soybean plants to express CV-N in seeds under the control of β-conglicinin α`promoter were produced. Analyses of R1 seeds showed that CV-N is expressed at 6% of total soluble seed protein, which is considerable high when compared to other similar publications. Additionally, transient expression of CV-N and mutated CV-N (Asn30Gln/Pro51Gly) in Nicotiana benthamiana leaves was tested with three different sorting signals. All tested constructions produced CV-N capable of binding to gp120, although the expression levels were about 0,1%. This work illustrates the advantage of producing recombinant proteins in seeds rather than in leaves.

ASSUNTO(S)

soja transgênica biologia molecular microbicida hiv ajanorrin

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