Expressão e distribuição da conexina 32 em fígados com fibrose experimentalmente induzida / Expression and distribution of connexin 32 in liver with experimentally induced fibrosis

AUTOR(ES)

Alexandro dos Santos Rodrigues

DATA DE PUBLICAÇÃO

2004

RESUMO

The connexin 32 (Cx32) is a proteic structure that constitute the channels that promote the cell communication by means of the gap junction (GJIC), allowing the diffusion of short cytoplasmic molecules from a cell to another. This work aimed to study these structures due to their importance in the hepatic metabolic processes. The hepatic fibrosis was triggered by the oral administration of dimethylnitrosamine (DMN) in the female rat Wistars twice a week in consecutive days during five weeks. The necropsy of these animals was carried out after the last drug administration. They presented a hepatic fibrosis state. The fibrotic material was submitted to the imunohistochemical analysis, which showed a preferencial presence of Cx32 in the cytoplasm, whereas in the control group the Cx32 was located at the membranes, in the junctional plaques. The molecular analysis showed a decrease of the genic expresson of the fibrotic material, however the proteic product wasn? t reduced in comparison with the control group as it was shown by western blot. We concluded that the fibrotic state introduced a disturbance in the intracellular distribution and genic expression of the connexin 32.

ASSUNTO(S)

conexina 32 hepatic fibrosis dimetilnitrosamina dimethylnitrosamine fibrose hepática connexin 32

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