Expression and Functional Properties of the Streptococcus intermedius Surface Protein Antigen I/II
AUTOR(ES)
Petersen, F.C.
FONTE
American Society for Microbiology
RESUMO
Streptococcus intermedius is associated with deep-seated purulent infections. In this study, we investigated expression and functional activities of antigen I/II in S. intermedius. The S. intermedius antigen I/II appeared to be cell surface associated, with a molecular mass of approximately 160 kDa. Northern blotting indicated that the S. intermedius NCTC 11324 antigen I/II gene was transcribed as a monocistronic message. Maximum expression was seen during the early exponential phase. Insertional inactivation of the antigen I/II gene resulted in reduced hydrophobicity during early exponential phase, whereas no effect was detected during mid- and late exponential phases. Binding to human fibronectin and laminin was reduced in the isogenic mutant, whereas binding to human collagen types I and IV and to rat collagen type I was not significant for either the wild type or the mutant. Compared to the wild type, the capacity of the isogenic mutant to induce interleukin 8 (IL-8) release by THP-1 monocytic cells was significantly reduced. The results indicate that the S. intermedius antigen I/II is involved in adhesion to human receptors and in IL-8 induction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=98542Documentos Relacionados
- Functional Variation of the Antigen I/II Surface Protein in Streptococcus mutans and Streptococcus intermedius
- Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus.
- A protein fragment of streptococcal cell surface antigen I/II which prevents adhesion of Streptococcus mutans.
- Ultrastructural localization of protein antigens I/II and III in Streptococcus mutans.
- Binding Properties of Streptococcus gordonii SspA and SspB (Antigen I/II Family) Polypeptides Expressed on the Cell Surface of Lactococcus lactis MG1363