Expression of bacteriophage M13 DNA in vivo. Localization of the transcription initiation and termination signal of the mRNA coding for the major capsid protein.
AUTOR(ES)
Rivera, M J
RESUMO
During the infection cycle of the filamentous bacteriophage M13 a phage specific RNA species is made which selectively directs in vitro the synthesis of the precursor of the major capsid protein encoded by gene VIII. This RNA is unstable (its mean half-life is 11 min) and is made in amounts representing at least 2% of the newly synthesized RN. Nucleotide sequence analysis have indicated that the synthesis of this RNA species is initiated and terminated at the same promoter (G0.18) and termination signal (T0.25) of the M13 genome as the 8S RNA species made in vitro under the direction of M13 replicative form DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342214Documentos Relacionados
- Initiation and termination signals for transcription in bacteriophage M13.
- A Possible Role for RNA Polymerase in the Initiation of M13 DNA Synthesis
- "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.
- Stability of bacteriophage phi X174-specific mRNA in vivo.
- Changing patterns in cytoskeletal mRNA expression and protein synthesis during murine erythropoiesis in vivo.