Expression of Major Capsid Protein VP-1 in the Absence of Viral Particles in Thymomas Induced by Murine Polyomavirus
AUTOR(ES)
Sanjuan, Norberto
FONTE
American Society for Microbiology
RESUMO
Thymomas induced by polyomavirus strain PTA in mice are known to express the major capsid protein VP-1. Since the expression of a late structural protein such as VP-1 is considered a sign of virus replication, the present work attempted to clarify the implication of the presence of this protein in tumor cells. Electron microscopy of tumors showed a striking absence of viral particles in the vast majority of the cells. However, immunoelectron microscopy of the same samples demonstrated intranuclear VP-1 in most cells despite the absence of viral particles. Very little infectious virus was recovered from tumors. A change in the electrophoretic mobility of VP-1 from thymomas was detected compared with VP-1 from productively infected cells. The data presented in this work prove that the expression of VP-1 in polyomavirus-induced tumors is not synonymous with the presence of infectious virus, suggesting a possible defect in viral encapsidation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=115915Documentos Relacionados
- Hydroxyproline in the major capsid protein VP1 of polyomavirus.
- Polyomavirus major capsid protein VP1 is modified by tyrosine sulfuration.
- Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1.
- Virion assembly defect of polyomavirus hr-t mutants: underphosphorylation of major capsid protein VP1 before viral DNA encapsidation.
- Synthesis, posttranslational modifications, and nuclear transport of polyomavirus major capsid protein VP1.
