Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2
AUTOR(ES)
Tao, Yumin
FONTE
National Academy of Sciences
RESUMO
Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that presumably help direct the T-strand to the plant nucleus. We identified a tomato cDNA clone, DIG3, that encodes a protein that interacts with the C-terminal region of VirD2. DIG3 encodes an enzymatically active type 2C serine/threonine protein phosphatase. Overexpression of DIG3 in tobacco BY-2 protoplasts inhibited nuclear import of a β-glucuronidase-VirD2 nuclear localization signal fusion protein. Thus, DIG3 may be involved in nuclear import of the VirD2 protein and, consequently, the VirD2/transferred DNA complex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387391Documentos Relacionados
- Overexpression of virD1 and virD2 genes in Agrobacterium tumefaciens enhances T-complex formation and plant transformation.
- Agrobacterium VirD2 protein interacts with plant host cyclophilins
- Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein
- Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco.
- Import of Agrobacterium T-DNA into Plant Nuclei: Two Distinct Functions of VirD2 and VirE2 Proteins