Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression
AUTOR(ES)
Marasco, Rosangela
FONTE
American Society for Microbiology
RESUMO
A newly identified bglH gene coding for a phospho-β-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the β-glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from −3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression of bglH expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107296Documentos Relacionados
- The Functional ccpA Gene Is Required for Carbon Catabolite Repression in Lactobacillus plantarum
- Structural characterization of the bglH gene encoding a beta-glucosidase-like enzyme in an endophytic Bacillus pumilus strain
- Carbon Catabolite Repression in Lactobacillus pentosus: Analysis of the ccpA Region
- Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression.
- Carbon Catabolite Repression Regulates Glyoxylate Cycle Gene Expression in Cucumber.
