Expression of toll-like receptor 2 and neutrophil chemotaxis in Behçet´s disease / Expressão de receptores toll-like 2 e função quimiotáxica de neutrófilos na doença de Behçet

AUTOR(ES)

Fabrício de Souza Neves

DATA DE PUBLICAÇÃO

2009

RESUMO

Expressions of toll-like receptor (TLR) 2, TLR4, CD14, CD114 and CD116 were assessed on polymorphonuclear (PMN) neutrophils and monocytes of patients with Behçets disease (BD). PMN chemotactic responses under different stimulations were also measured. The objective was to determine if BD PMN chemotaxis may be overstimulated by lipoteichoic acid (LTA) from gram-positive bacteria. Receptor expressions were measured by flow cytometry and PMN chemotaxis was assessed in a Boyden chamber. Only TLR2 expression was higher on monocytes of the BD group than in control group. On PMNs, however, TLR2 expression was similar in both groups and, when stimulated with LTA, BD PMN cells showed chemotactic responses similar to the controls. These cells only exhibited increased chemotaxis when incubated with plasma. In conclusion, isolated BD PMN did not overreact to LTA, and its hyperchemotaxis is strictly dependent on soluble stimulating factors

ASSUNTO(S)

antigens cd14 toll-like receptor 2 chemotaxis monócitos receptor 4 toll-like síndrome de behçet/fisiopatologia neutrophils behçet syndrome/physiopathology neutrófilos monocytes receptor 2 toll-like quimiotaxia toll-like receptor 4 antígenos cd14

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