Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori

AUTOR(ES)
FONTE

International Union of Crystallography

RESUMO

Glyceraldehyde-3-phosphate dehydrogenase A has been cloned, expressed and purified. Apoprotein crystals have been grown which diffracted to 1.75 Å resolution and belonged to space group P21; holo crystals were grown in the presence of NADP, diffracted to 2.6 Å resolution and belonged to space group P32.

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