Extracellular antigens of Micropolyspora faeni grown in synthetic medium.
AUTOR(ES)
Kurup, V P
RESUMO
Extracellular antigens were prepared by growing Micropolyspora faeni in a new synthetic medium (SMSL), and this antigenic preparation was compared with antigens obtained by the double-dialysis method. These two preparations were tested against pooled sera from farmer's lung patients and rabbits immunized with these antigens by the agar-gel double-diffusion, immunoelectrophoresis, and two-dimensional immunoelectrophoresis methods. The immunological cross-reactivity between M. faeni strains of diverse origin and between other species of thermophilic actinomycetes associated with hypersensitivity pneumonitis was also investigated. The usefulness of two-dimensional immunoelectrophoresis in the comparison and standardization of antigens is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=421410Documentos Relacionados
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