Extragenic Suppressors of Nudc3, a Mutation That Blocks Nuclear Migration in Aspergillus Nidulans

AUTOR(ES)

Chiu, Y. H.

RESUMO

Nuclear migration plays an important role in the growth and development of many organisms including the filamentous fungus Aspergillus nidulans. We have cloned three genes from A. nidulans, nudA, nudC, and nudF, in which mutations affect nuclear migration. The nudA gene encodes the heavy chain of cytoplasmic dynein. The nudC gene encodes a 22-kD protein. The nudF gene was identified as an extracopy suppressor of the temperature sensitive (ts(-)) nudC3 mutation. The nudC3 mutation substantially decreases the intracellular concentration of the nudF protein at restrictive temperature. This is restored toward the normal level by an extra copy of nudF. To identify other genes whose products interact directly or indirectly with the NUDC protein, we have isolated a set of extragenic suppressors of the nudC3 temperature-sensitive mutation. Genetic analysis of 16 such extragenic suppressors showed them to represent nine different genes, designated sncA-sncI (for suppressor of nudC). sncA-sncH were either dominant or semidominant in diploids homozygous for nudC3 and heterozygous for the snc mutations. All of the suppressors reversed the ts(-) phenotype of nudC3 by restoring the intracellular concentration of the NUDF protein.

Documentos Relacionados

• Extragenic Suppressors of a Dynein Mutation That Blocks Nuclear Migration in Aspergillus Nidulans
• Genetic and Molecular Analysis of a Trna(leu) Missense Suppressor of Nudc3, a Mutation That Blocks Nuclear Migration in Aspergillus Nidulans
• An Alpha Tubulin Mutation Suppresses Nuclear Migration Mutations in Aspergillus Nidulans
• Deletion of nudC, a nuclear migration gene of Aspergillus nidulans, causes morphological and cell wall abnormalities and is lethal.
• Genetic Analysis of Suppressors of the Vea1 Mutation in Aspergillus Nidulans