F Pilus as f+ Antigen

AUTOR(ES)

Ishibashi, Masahide

RESUMO

Specific aggregate formation of F pili was observed, by electron microscopy, in a mixture of male Escherichia coli (or of isolated F pili) and anti-f+ serum. Cellular appendages other than F pili never showed such aggregation when mixed with anti-f+ serum. The f+ agglutinability of male cells, as well as F piliation, was sensitive to mechanical agitation. The f+ agglutination was inhibited when appropriate numbers of phage M12, capable of attaching to F pili, were mixed with the male culture before the addition of anti-f+ serum. Correlation between f+ agglutinability and the extent of F piliation was observed. It was concluded that the F pilus is the structure of the f+ antigen and is responsible for f+ agglutination.

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