Factor 420-dependent tyridine nucleotide-linked hydrogenase system of Methanobacterium ruminantium.
AUTOR(ES)
Tzeng, S F
RESUMO
Methanobacterium ruminantium was shown to possess a nicotinamide adenine dinucleotide phosphate (NADP)-linked factor 420 (F420)-dependent hydrogenase system. This system was also shown to be present in Methanobacterium strain MOH. The hydrogenase system of M. ruminantium also links directly to F420, flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), methyl viologen, and Fe-3 plus. It has a pH optimum of about 8 and an apparent Km for F420 of about 5 x 10-6 M at pH 8 when NADP is the electron acceptor. The F420-NADP oxidoreductase activity is inactive toward nicotinamide adenine dinucleotide (nad) and no NADPH:NAD or FADH2(FMNH2):NAD transhydrogenase system was detected. Neither crude ferredoxin nor boiled crude extract of Clostridium pasteuranum could replace F420 in the NADP-linked hydrogenase reaction of M. ruminantium. Also, neitther F420 nor a curde "ferredoxin" fraction from M. ruminantium extracts could substitute for ferredoxin in the pyruvate-ferredoxin oxidoreductase reaction of C. pasteurianum.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285629Documentos Relacionados
- Factor 420-dependent pyridine nucleotide-linked formate metabolism of Methanobacterium ruminantium.
- PYRIDINE NUCLEOTIDE-LINKED REACTIONS OF PSEUDOMONAS NATRIEGENS
- Pyridine nucleotide-linked oxidation of methanol in methanol-assimilating yeasts.
- Purification and Separation of Pyridine Nucleotide-Linked Dehydrogenases by Affinity Chromatography Techniques
- THE DISTRIBUTION IN HIGHER PLANTS OF TRIPHOSPHOPYRIDINE NUCLEOTIDE-LINKED ENZYME SYSTEMS CAPABLE OF REDUCING GLUTATHIONE