Factors affecting the palmitoyl-coenzyme A desaturase of Saccharomyces cerevisiae.
AUTOR(ES)
Klein, H P
RESUMO
The activity and stability of the palmitoyl-coenzyme A (CoA) desaturase complex of Saccharomyces cerevisiae was influenced by several factors. Cells, grown nonaerobically and then incubated with glucose, either in air or under N2, showed a marked increase in desaturase activity. Cycloheximide, added during such incubations, prevented the increase in activity, suggesting de novo synthesis. The stability of the desaturase from cells grown nonaerobically was affected by subsequent treatment of the cells; enzyme from freshly harvested cells, or from cells that were then shaken under nitrogen, readily lost activity upon washing or during density gradient analysis, whereas aerated cells, in the presence or absence of glucose, yielded stable enzyme preparations. The loss of activity in nonaerobic preparations could be reversed by adding soluble supernatant from these homogenates and could be prevented by growing the cells in the presence of palmitoleic acid and ergosterol, but not with several other lipids tested.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235959Documentos Relacionados
- Interaction of mycobacterial polymethylpolysaccharides with paranaric acid and palmitoyl-coenzyme A: Structural specificity and monomeric dissociation constants
- Mutations in trans-acting factors affecting mRNA decapping in Saccharomyces cerevisiae.
- Effects of metals on acetyl-coenzyme A carboxylase activity of Saccharomyces cerevisiae.
- Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae.
- Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.