Fate of microfilaments in vero cells infected with measles virus and herpes simplex virus type 1.
AUTOR(ES)
Bedows, E
RESUMO
In herpes simplex virus type 1-infected Vero cells, reorganization of microfilaments was observed approximately 4 h postinfection. Conversion of F (filamentous) actin to G (globular) actin, as assessed by a DNase I inhibition assay, was continuous over the next 12 to 16 h, at which time a level of G actin of about twice that observed in uninfected cells was measured. Fluorescent localization of F actin, using 7-nitrobenz-2-oxa-1,3-diazole (NBD)-phallacidin, demonstrated that microfilament fibers began to diminish at about 16 to 18 h postinfection, roughly corresponding to the time that G actin levels peaked and virus-induced cytopathology was first observable. In measles virus-infected cells, no such disassembly of microfilaments occurred. Rather, there was a modest decrease in G actin levels. Fluorescent localization of F actin showed that measles virus-infected Vero cells maintained a complex microfilament network characterized by fibers which spanned the entire length of the newly formed giant cells. Disruption of microfilaments with cytochalasin B, which inhibits measles virus-specific cytopathology, was not inhibitory to measles virus production at high multiplicities of infection (MOI) but was progressively inhibitory as the MOI was lowered. The carbobenzoxy tripeptide SV-4814, which inhibits the ability of Vero cells to fuse after measles virus infection, like cytochalasin B, inhibited measles virus production at low MOI but not at high MOI. Thus, it appears that agents which affect the ability of Vero cells to fuse after measles virus infection may be inhibitory to virus production and that the actin network is essential to this process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368587Documentos Relacionados
- Deoxyribopyrimidine triphosphatase activity specific for cells infected with herpes simplex virus type 1.
- Influence of acyclovir and bucyclovir on nucleotide pools in cells infected with herpes simplex virus type 1.
- Viral DNA synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1.
- Effector cell involved in cell-mediated cytotoxicity to cells infected with herpes simplex virus type 1.
- Differential stability of host mRNAs in Friend erythroleukemia cells infected with herpes simplex virus type 1.