Ferramenta biofotonica integrada para manipulações e microscopias confocais / Integrates biophotonic tool for manipulations and confocal microscopies
AUTOR(ES)
Andre Alexandre de Thomaz
DATA DE PUBLICAÇÃO
2007
RESUMO
The research in biomedical photonics is clearly evolving in the direction of the understanding of biological processes at the cell level. The spatial resolution to accomplish this task practically requires photonics tools. However, an integration of different photonic tools and a multimodal and functional approach will be necessary to access the mechanical and biochemical cell processes. This way we can observe mechanicaly triggered biochemical events or biochemicaly triggered mechanical events, or even observe simultaneously mechanical and biochemical events triggered by other means, e.g. electricaly. One great advantage of the photonic tools is its easiness for integration. Therefore, we developed such integrated tool by incorporating single Optical Tweezers with Confocal Single and Multiphoton Microscopies. This system can perform 2-photon excited fluorescence and Second Harmonic Generation microscopies together with optical manipulations. Force, elasticity and viscosity measurements of stretched membranes can be followed by real time confocal microscopies. Also opticaly trapped living protozoas, such as Trypanosoma cruzi. Integration with CARS microscopy is under way. We will show several examples of the use of such integrated instrument and its potential to observe mechanical and biochemical processes at cell level
ASSUNTO(S)
microscopia confocal imagens oticas confocal microscopy optical tweezers second harmonic generation microscopy multiphoton confocal microscopy microscopia confocal multifoton microscoía de geração de segundo harmonico pinças oticas optical images
