Filter replicas and permanent collections of recombinant DNA plasmids.
AUTOR(ES)
Gergen, J P
RESUMO
A permanent, ordered collection of 23,000 recombinant DNA plasmids containing Drosophila melanogaster DNA has been established. Simple and practical methods for storing and manipulating this collection were developed. In addition, an improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed. These filter replicas are suitable for nucleic acid hybridization screens of recombinant DNA colinies and each filter replica can be used for many (greater than 5) successive screens. The kinetics of this hybridization reaction were examined and allow design of experiments that detect colony complementarity to a nucleic acid that is 0.5% of the hybridization probe.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342374Documentos Relacionados
- Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
- DNA gyrase improves DNA transformation of E.coli cells with large recombinant plasmids.
- Detection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.
- A cloned immunoglobulin cDNA fragment enhances transposition of IS elements into recombinant plasmids.
- A rapid procedure for the screening of recombinant plasmids.
