Fine-Structure Mapping of the Acetamidase Structural Gene and Its Controlling Region in ASPERGILLUS NIDULANS
AUTOR(ES)
Hynes, M. J.
RESUMO
A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not produce detectable inactive gene product, was found to be inseparable by recombination from the "up-promoter" mutation amdI18 and was located outside of the apparent amdS coding region. The cis-dominant mutation, amdI9, was also located at this end of the gene. This work, therefore, provides evidence for the separation of a eukaryotic gene into controlling and structural regions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1216838Documentos Relacionados
- Fine-Structure Mapping and Mutational Studies of Gene Controlling Yeast Cytochrome c
- Fine-Structure Mapping by Transformation in the Tryptophan Region of Bacillus subtilis
- Fine-structure mapping of the three mouse alpha-fetoprotein gene enhancers.
- Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.
- Chromatin fine-structure mapping of the goat beta F gene in fetal erythroid tissue.
