Fluid accumulation in mouse ligated intestine inoculated with Clostridium perfringens enterotoxin.
AUTOR(ES)
Yamamoto, K
RESUMO
Clostridium perfringens enterotoxin, when inoculated into the ligated intestinal loop of mice, caused marked distension due to fluid accumulation. The increase in weight of the intestinal loop was proportional to the log dose of enterotoxin within a range from 1 to 16 micrograms. The fluid accumulation was arrested by washing the loop with saline or by injection of the specific anti-enterotoxin serum into the loop 5 or even 30 min after inoculation of the enterotoxin. A significant increase in weight of the loop was found as early as 10 min after inoculation of the toxin. These results may suggest that entergotoxin is neither bound firmly to the mucosal membrane nor permeates into the cells of the intestinal wall. The mouse intestinal loop test is economical, simple to perform, and applicable for quantitative determination of the enteropathogenic activity of C. perfringens enterotoxin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243184Documentos Relacionados
- Deletion analysis of the Clostridium perfringens enterotoxin.
- Affinity chromatography purification of Clostridium perfringens enterotoxin.
- Preparative polyacrylamide gel electrophoresis purification of Clostridium perfringens enterotoxin.
- Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.
- Production and characterization of monoclonal antibodies to Clostridium perfringens enterotoxin.
