Foliculogênese, maturação nuclear e citoplasmática de oócitos bubalinos - Bubalus bubalis : uma análise estrutural

AUTOR(ES)

Rafael Gianella Mondadori

DATA DE PUBLICAÇÃO

2008

RESUMO

The rusticity and milk production with high market value make possible to buffalos be bred in low or highly technified production system. On the other way, some species inherent characteristics make difficult to apply reproductive biotechniques to multiply animals (mainly females) with high genetic potential. Considering this, the present research established the ultrastructural characteristics of female gamete development. To achieve the objective, buffalo cumulus-oocyte complexes (COCs) and ovaries were properly processed to light and transmission electron microscopy. The buffalo oocyte and preantral follicles were smaller than bovine ones and have higher lipid content (progressive accumulation). The specie also shows a lower number of preantral follicles in the ovaries, as well as, the number of primary is superior to primordial and secondary follicles. Other described factors which affects the application of ovum pick-up technique, other than the biosecurity, is the apparently fragile aspect of zona pellucida and the presence of fissures in its surface. Buffalo oocyte cytoplasmic maturation was characterized by organelle migration to cortical region. In addition, as the maturation progress, the perivetilline space increased in size and it is possible to observe a progressive reduction on granulosa cells and oocyte connections. In conclusion, the present research confirmed that the chemical modifications possibly suffered by oocyte lipids during in vitro maturation period, cause an alteration in its ultrastructural aspect. It is also important to note that is possible, with some modifications on in vitro embryo production systems, to produce buffalo embryo in seasonal anoestrous.

ASSUNTO(S)

morphometry microscopia eletrônica de transmissão in vitro maturation maturação in vitro microscopia óptica população de folículos pré-antrais transmission electron microscopy morfometria optical microscopy preantral follicles population biologia molecular

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