Four highly stable eukaryotic mRNAs assemble 3′ untranslated region RNA–protein complexes sharing cis and trans components

AUTOR(ES)

Holcik, Martin

FONTE

The National Academy of Sciences of the USA

RESUMO

Assembly of a sequence-specific RNA–protein complex on the 3′ untranslated region (3′UTR) of human α-globin mRNA (α-complex) correlates with mRNA stabilization. Here we map a limited segment of the α-globin 3′UTR that is both necessary and sufficient for α-complex formation. The sequence of this binding region identifies three additional, highly stable mRNAs that share closely related, pyrimidine-rich cis-motifs in their respective 3′UTRs. Each mRNA assembles a sequence-specific ribonucleoprotein complex at this conserved region. These complexes are structurally related, and each contains a 39-kDa cytoplasmic poly(C) binding protein previously demonstrated to be essential to formation of the α-complex. These observations indicate the existence of a general determinant for stabilization of eukaryotic mRNAs.

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