Functional analysis of the murine IgH enhancer: evidence for negative control of cell-type specificity.

AUTOR(ES)

Kadesch, T

RESUMO

We have carried out a mutational analysis of the mouse IgH enhancer. Consistent with previous reports, deletions extending from either the 5' side or the 3' side of the enhancer fail to reveal distinct boundaries which define enhancer function in lymphoid cells. Interestingly, internal point mutations and deletions within the "enhancer core" regions fail to identify any necessary functional role for these conserved elements. When tested in CV1 cells, which do not normally respond to the IgH enhancer, certain deletions exhibit significant enhancer activity. We take these findings to indicate that the functional domains of the IgH enhancer are complex and that cell type specificity is defined in part by negative factors present in non-lymphoid cells.

Documentos Relacionados

• Properties of the human hepatitis B virus enhancer: position effects and cell-type nonspecificity.
• Evidence for transient requirement of the IgH enhancer.
• Cell-type specificity of short-range transcriptional repressors
• A T cell controlled molecular pathway regulating the IgH locus: CD40-mediated activation of the IgH 3' enhancer.
• Lymphoid-specific transcriptional activation by components of the IgH enhancer: studies on the E2/E3 and octanucleotide elements.