Functional diversity of gro gene expression in human fibroblasts and mammary epithelial cells.
AUTOR(ES)
Anisowicz, A
RESUMO
Previous studies of gro and related genes that are overexpressed in transformed fibroblasts suggest that gro may encode a specific growth regulator. However, DNA and protein sequence comparisons reveal relatedness to platelet factor 4 and other proteins involved in the inflammatory response. In this paper, both growth-related and cytokine-induced responses in gro gene expression are described. Human foreskin fibroblasts are shown to express approximately 10-fold elevated gro, myc, and fos mRNAs in response to serum and to phorbol 12-myristate 13-acetate stimulation, with early response kinetics indicative of growth regulation. In response to interleukin 1, however, in growing cells gro mRNA is elevated at least 100-fold but myc remains constant and fos is not expressed, suggesting a second regulatory pathway. In normal cultured mammary epithelial cells, gro is constitutively expressed, and elevated mRNA levels are induced by phorbol 12-myristate 13-acetate, but not by interleukin 1. However, most carcinoma cell lines examined do not express gro mRNA, suggesting a third function of gro as a negative growth regulator in epithelial cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=282824Documentos Relacionados
- Distinctive gene expression patterns in human mammary epithelial cells and breast cancers
- Expression of Mn-Superoxide Dismutase Gene in Nontumorigenic and Tumorigenic Human Mammary Epithelial Cells
- Transfection of beta-casein chimeric gene and hormonal induction of its expression in primary murine mammary epithelial cells.
- Down-regulation of a calmodulin-related gene during transformation of human mammary epithelial cells.
- Biological action of epidermal growth factor and its functional receptors in normal mammary epithelial cells.