Functional identification and genomic structure of Paracoccidioides brasiliensis nuclear genes associated to cytochrome c oxidase activity. / Identificação funcional e estrutura genômica de genes nucleares associados à atividade da citocromo C oxidase de Paracoccidioides brasiliensis.
AUTOR(ES)
Simone Cristina Borges Bandeira
DATA DE PUBLICAÇÃO
2009
RESUMO
Paracoccidioides brasiliensis is the ethologic agent of the paracoccioidomycose, a systemic mycosis prevalent in Latin American. This is a thermo dimorphic fungus living in the mycelium form at room temperature (25ºC) and in the yeast form between 35 ºC e 37 ºC. In this work we identified and study fifteen (15) new P. brasiliensis genes involved in the mitochondrial cytochrome c oxidase expression (COX). The genes PbCOX6, PbCOX17, PbCOX19 e PbOXA1 were able to functionally replace its homologues in the respective S. cerevisiae null mutant. All P. brasiliensis genes analyzed have their expression modulated during the morphologic transition from mycelium to yeast as well as from yeast to mycelium. This observation highlighted the importance of the respiration related genes in the fungus pathogenicity establishment. Genomic structure analyzes of these genes confirmed introns presence as well as evidenced the alternative splicing occurrence. It was demonstrated bioactive compounds siderofore production in P. brasiliensis been detected siderofore secretion in the solids and liquid medium.
ASSUNTO(S)
microbiologia fungos respiração celular saccharomyces microbiology fungi paracoccidioides genética molecular paracoccidioides saccaharomyces molecular genetics cellular respiration
Documentos Relacionados
- Molecular detection and identification of Paracoccidioides brasiliensis.
- Caracterização de marcadores de virulência em Paracoccidioides brasiliensis.
- Identificação e estudo funcional de genes associados com doenças neurológicas
- Effect of detergents on membrane-associated glucan synthetase from Paracoccidioides brasiliensis.
- Atividade citocromo C oxidase em espermatozoide bovinos.