Functional Interactions between the p35 Subunit of the Arp2/3 Complex and Calmodulin in Yeast
AUTOR(ES)
Schaerer-Brodbeck, Claudia
FONTE
The American Society for Cell Biology
RESUMO
The end9-1 (arc35-1) mutant was identified as an endocytosis mutant and is a mutant allele of ARC35 that encodes a subunit of the Arp2/3 complex. As for other mutants in the Arp2/3 complex, arc35-1 is defective for endocytosis and organization of the actin cytoskeleton. Both defects can be suppressed by overexpression of calmodulin. Analysis of a collection of temperature-sensitive cmd1 mutants for their ability to suppress either the endocytic defect and/or the actin defect indicates that the two defects are tightly coupled. We demonstrate that Arc35p and Cmd1p interact and that Arc35p is required for cortical localization of calmodulin. This is the first report linking Arp2/3 complex function with calmodulin through which it exercises at least one of its endocytic functions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=14835Documentos Relacionados
- A Mutant of Arp2p Causes Partial Disassembly of the Arp2/3 Complex and Loss of Cortical Actin Function in Fission Yeast
- Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast
- Involvement of the Arp2/3 Complex and Scar2 in Golgi Polarity in Scratch Wound Models
- DISTORTED3/SCAR2 Is a Putative Arabidopsis WAVE Complex Subunit That Activates the Arp2/3 Complex and Is Required for Epidermal Morphogenesis
- The Putative Arabidopsis Arp2/3 Complex Controls Leaf Cell Morphogenesis1[w]
