Functional organization of plasmid pKM101.

AUTOR(ES)

Langer, P J

RESUMO

Tn5 insertion mutants and in vitro-generated deletion mutants of the mutagenesis-enhancing plasmid pKM101 have been used to identify several genetic regions on the pKM101 map. In clockwise order on the pKM101 map are: (i) the bla gene, coding for a beta-lactamase; (ii) the Slo region, responsible for retarding cell growth on minimal medium; (iii) the tra genes, enabling pKM101 to transfer conjugally; (iv) sensitivity to IKe phage (this function[s] maps within the tra region); (v) the muc gene(s), responsible for enhancing ultraviolet light and chemically induced mutagenesis in the cell; and (vi) the Rep region, essential for plasmid replication. The muc gene(s) and the Rep region are contained in a deoxyribonucleic acid region bounded by inverted repeated sequences.

Documentos Relacionados

• Conjugal transfer system of the IncN plasmid pKM101.
• Entry exclusion determinant(s) of IncN plasmid pKM101.
• Fertility inhibition of RP1 by IncN plasmid pKM101.
• Characterization of an endonuclease associated with the drug resistance plasmid pKM101.
• Genetic and biochemical analysis of an endonuclease encoded by the IncN plasmid pKM101.