Functional reconstitution of photosynthetic reaction centers in planar lipid bilayers

AUTOR(ES)

Schönfeld, M.

RESUMO

Planar lipid bilayers containing reaction centers from Rhodopseudomonas sphaeroides R-26 were formed by apposing two reaction center—lipid monolayers formed from a reaction center—lipid complex in hexane. Secondary donors (cytochrome c) and acceptors (ubiquinone-0) were added on opposite sides of the membrane. Upon illumination, this system generated transient and steady-state voltages and currents. The wavelength dependence of the photoresponse matched the absorption spectrum of reaction centers. A simple model based on the transfer of charges across the membrane that explains the salient features of the photoresponse is presented.

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