Functional RNA polymerase II promoters in solitary retroviral long terminal repeats (LTR-IS elements).
AUTOR(ES)
Köhrer, K
RESUMO
LTR-IS elements are middle repetitive sequences in the mouse genome with structural features of solitary retroviral LTRs. In order to get some insight in the possible functional role of these sequences the promotor activity of two LTR-IS representatives differing by 105 bp in their U3 region was investigated. Gene fusions between LTR-IS sequences and the bacterial gene coding for chloramphenicol acetyl transferase (CAT) were transfected into mouse 3T6 cells and the expression of CAT was measured. It is shown that the LTR-IS sequences represent weak RNA polymerase II promoters which require enhancement by cis-or trans-activating factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341180Documentos Relacionados
- Evidence that a major class of mouse endogenous long terminal repeats (LTRs) resulted from recombination between exogenous retroviral LTRs and similar LTR-like elements (LTR-IS).
- Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats.
- Evidence for mobility of a new family of mouse middle repetitive DNA elements (LTR-IS).
- Transcriptional interaction between retroviral long terminal repeats (LTRs): mechanism of 5' LTR suppression and 3' LTR promoter activation of c-myc in avian B-cell lymphomas.
- Localization of active promoters for eucaryotic RNA polymerase II in the long terminal repeat of avian sarcoma virus DNA.
