Functionally Abnormal Na+-K+ Pump in Erythrocytes of a Morbidly Obese Patient
AUTOR(ES)
Deluise, Mario
RESUMO
The Na+-K+ pump in the erythrocytes of a mordibly obese patient shows a unique constellation of functional abnormalities. The number of pump units, measured by [3H]ouabain binding to intact cells, as well as the enzymatic activity of the (Na+-K+)-dependent ATPase in erythrocyte membranes were found to be markedly increased compared with control cells (18-fold and 14-fold, respectively). There was a concomitant fivefold increase in the rate of pump-mediated uptake of 86Rubidium (a K analogue); this was balanced by an increased rate of 86Rb efflux. In striking contrast to normal cells, however, a major portion of this efflux (80%) was inhibited by ouabain, and thus appeared to be mediated by the Na+-K+ pump.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=371166Documentos Relacionados
- Properties of the Na+-K+ pump in human red cells with increased number of pump sites.
- Lithium efflux through the Na/K pump in human erythrocytes
- Potassium transport across rat alveolar epithelium: evidence for an apical Na+-K+ pump.
- Relation between Na+-K+ pump, Na+ activity and force in strophanthidin inotropy in sheep cardiac Purkinje fibres.
- Occlusion of cobalt ions within the phosphorylated forms of the Na+-K+ pump isolated from dog kidney.