Further Characterization of Tissue Distribution and Metabolism of [14C]Aflatoxin B1 in Chickens1

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The distribution and metabolism of [14C]aflatoxin B1 in chicken tissues were further investigated. Previously dried and frozen ethyl acetate extracts of liver, heart, gizzard, breast, leg, blood, and fecal samples were obtained from either layer or broiler chickens fed subclinical levels of [14C]aflatoxin B1. Treatment of these extracts with either carboxypeptidase A, leucine aminopeptidase, pepsin, or trypsin revealed that an average of 50% of the 14C detected in the acetate extracts was a liberated peptide (or amino acid) conjugate of [14C]aflatoxin B2a. When a prepared standard of B2a was made by incubation of B1 with cold dilute aqueous HCl, the Rf values and absorbance maxima were identical with those of the tissue extracts after enzymatic treatment.

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