Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

AUTOR(ES)

Gomes, Luciana I, Marques, Letícia HS, Enk, Martin J, Coelho, Paulo Marcos Z, Rabello, Ana

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2009-12

RESUMO

A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.

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