GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis
AUTOR(ES)
Chang, Aaron N.
FONTE
The National Academy of Sciences
RESUMO
The function of GATA transcription factors in diverse developmental contexts depends in part on physical interaction with cofactors of the Friend of GATA (FOG) family. However, previous studies indicate that FOG-1 may play a GATA-1-independent role in early megakaryopoiesis, suggesting that FOG proteins might act in a GATA factor-independent manner. Here, we have generated mouse knock-in (KI) mutants harboring a critical valine-to-glycine substitution in the amino-terminal zinc fingers of GATA-1 and GATA-2 to ablate FOG interaction. In contrast to male GATA-1KI (GATA-1 is located on the X-chromosome) or GATA-2KI/KI mice, compound GATA-1KI GATA-2KI/KI mutant mice display complete megakaryopoietic failure, a phenocopy of FOG-1−/− mice. We conclude that FOG-1 requires an interaction with either GATA-1 or -2 as part of its essential role in early megakaryopoiesis. On the basis of these and previous reports, we infer that GATA factor dependence is a critical aspect of FOG protein function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123124Documentos Relacionados
- FOG-2: A novel GATA-family cofactor related to multitype zinc-finger proteins Friend of GATA-1 and U-shaped
- The multitype zinc-finger protein U-shaped functions in heart cell specification in the Drosophila embryo
- Association of erythroid transcription factors: complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1.
- Expression of the Zinc-Finger Antiviral Protein Inhibits Alphavirus Replication
- A novel four zinc-finger protein targeted against p190BcrAbl fusion oncogene cDNA: utilisation of zinc-finger recognition codes