Gene Control in Germinal Differentiation: Rnf6, a Transcription Regulatory Protein in the Mouse Sertoli Cell
AUTOR(ES)
Lopez, Pascal
FONTE
American Society for Microbiology
RESUMO
In mouse Sertoli cells, transcription of the Inha gene encoding the α subunit of inhibin, which acts locally as a tumor suppressor, is down-regulated in tumors and in normal cells during aging. Previous studies suggested that regulation of Inha transcription involves the binding of a protein(s) to a repeat of the GGGGC motif in the promoter. Expression screening identified a cDNA encoding a protein that binds this sequence. Of the RING-H2 family, it is the mouse homologue of a human protein of unknown function, RNF6. The mouse gene, Rnf6, is predominantly expressed in two interacting cell types of the testis, Sertoli cells and pachytene spermatocytes. In Sertoli cells, it colocalizes with the PML and Daxx proteins in punctate nuclear bodies. In transient and stable transfectants, Rnf6 expression from a heterologous promoter increased the expression of reporter genes driven by the Inha promoter. In a Sertoli tumor cell line in which expression of both Inha and Rnf6 was reduced, reexpression of the latter restored the level of Inha while, concomitantly, the cells reverted to normal growth control in culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=133796Documentos Relacionados
- Developmental control of transcription of a retina-specific gene, QR1, during differentiation: involvement of factors from the POU family.
- Embryonic stem cell differentiation: A chromatin perspective
- Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract.
- Chromosome Behavior in Cell Differentiation: A Field Ripe for Exploration?
- Laminin-induced retinoblastoma cell differentiation: possible involvement of a 100-kDa cell-surface laminin-binding protein.